Antibodies to detect LINE-1-encoded proteins ORF1p antibodies We developed a mouse monoclonal antibody, 4H1, that recognizes epitope MENDFDELRE on human LINE-1 ORF1p. The reagent can be purchased from Millipore Sigma (catalog no. MABC1152).
ORF2p antibodies ORF2p antibodies are currently being prepared for commercial production by Abcam. In the meantime, our lab has limited ability to distribute these reagents. Investigators are welcome to contact us for updates.
Reference: LINE-1 ORF2p expression is nearly imperceptible in human cancers. Ardeljan D, Wang X, Oghbaie M, Taylor MS, Husband D, Deshpande V, Steranka JP, Gorbounov M, Yang WR, Sie B, Larman HB, Jiang H, Molloy KR, Altukhov I, Li Z, McKerrow W, Fenyö D, Burns KH, LaCava J. Mob DNA. 2019 Dec 31;11:1. doi: 10.1186/s13100-019-0191-2.
Plasmids Our lab has deposited LINE-1 expression plasmids at Addgene.
Software for Quantifying Interspersed Repeat Expression (SQuIRE) Our lab has developed a computational approach for evaluating interspersed repeat expression in RNAseq data. This can be used to identify L1, ERV, and Alu expression that may be involved in viral mimicry.